That question isn't a 'deal breaker' by any means.
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2.2 Is this the first time you have applied for a visa or extension of stay in one of the above categories (including previous leave granted as a fiancé(e) or proposed civil partner) with your current sponsor?
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Either a visa application or application for extension of stay under the category of Spouse (which happens to include fiance(e) or proposed civil partner)
Don't forget with the same sponsor.
Your wife already applied for a visa as spouse and with you as her sponsor so this is her second time
Tick the
no box. It's not her first time.
The first time was in Manila